There are several types of eye infections caused by a variety of microorganisms.For effective diagnosis and treatment of these infections is extracted and laboratory testing of samples taken from the patient.There is a wide range of bacterial infections of the eyes - from the relatively harmless, "barley" to a much more dangerous orbit of cellulite, which is a threat to sight.Accurate diagnosis and treatment of bacterial eye infections can not do without the participation of the microbiology laboratory.Eye diseases Infectious diseases of the eye - how to identify them?
Types of eye infections
Infections century - the most common infectious diseases of the century is blepharitis (prolonged itching, redness and flaking century) and "barley" (infection of the follicle, or root of the eyelashes).Conjunctivitis - an infectious inflammation of the conjunctiva - the membrane covering the front of the eye.More common in infants, young children and the elderly.Symptoms include redness, swelling of the
keratitis - infection of the cornea - the transparent central part of the eyeball.Infection can occur after trauma or severe corneal conjunctivitis.Infections of the orbit (the area of the skull that contains the eyeball).Inflammation may result from infection of the sinuses, periorbital area of injury or inflammation of subcutaneous tissue (cellulite).The intraocular infection (endophthalmitis) - can develop after surgery or eye injury.
possibility of secondary damage in the eyes of the spread of infection from the source, which is located in any part of the body.For most of eye infections are responsible only a few types of bacteria.Staphylococcus aureus causes infections eyelids, conjunctivitis babies n endophthalmitis.Streptococcus pneumoniae is a cause of pneumonia, meningitis and infections of the eye.Haemophilus influenzae can cause conjunctivitis and eye socket cellulitis, especially in children under five.Pseudomonas aeruginosa may infect the eye after injury or surgery.Neisseria gonorrhoeae - causes gonorrhea - a disease transmitted through sexual contact.To microbiological laboratory can provide accurate results, you need to properly implement the collection of material and immediately send it to the study of eye diseases, infectious diseases of the eye.
doctor may be directed to study the detection of eye diseases, infectious diseases of the eye, the following types of material samples: smears - intake of eye secretions, sterile swabs.These swabs are sent to the lab by placing a special fluid that supports the viability of the bacteria, but does not allow them to proliferate.Smears handy in cases of superficial ocular infections, but with deeper localization of the application of this method is limited.Pus is sent to the microbiology laboratory in a sterile container;blood - in the case of severe infection is carried out blood sampling, since the germs that caused the infection process, are able to penetrate into the bloodstream.Samples of the material sent to the microbiology laboratory where are used multiple methods of isolation and identification of different species of microbes that cause infections of the eye.In this task, technicians assist clinical data.
When swab or a sample of pus first enters the lab, conducted its Gram stain on a slide, which is then placed under a microscope and examined for the presence of bacteria and white blood cells - early signs of infection.Various micro-organisms in the eye diseases, infectious diseases of the eyes look different after the Gram stain.Staphylococcus aureus and Streptococcus pneumoniae -grampolozhitelnye cocci (bacteria circular shape) and different groups of bacteria located on the slide.Haemophilus influenzae, and Pseudomonas aeruginosa -gramotritsatelnye rods (rod-shaped bacteria).Neisseria gonorrhoeae - Gram-negative cocci, forming a pair in white blood cells.
isolation of microorganisms
typical form of certain bacteria in eye diseases infectious diseases of the eye, allowing the technician in the study of stained smears get an accurate picture of the nature of microorganisms.On this basis, he selects the most suitable medium for culturing.The doctor receives a preliminary result and selects the appropriate antibiotic effective against this type of bacteria.Later, the doctor is the final result and, if necessary, correcting therapy.In order to isolate and identify the bacteria causing infectious diseases of the eye, swabs and pus samples inoculated into dishes containing various nutrient medium.For blood cultures are widely used blood agar plates, because they are growing well, most bacteria.Overnight plates were placed in an incubator, the temperature and the concentration of oxygen or carbon dioxide which can be varied to promote the growth of certain bacteria.
blood culture samples were incubated overnight - in the presence of oxygen and in the absence of oxygen.The next day, a small portion of the sample taken for Gram stain, and the cup is left for further incubation and identification of microbial culture.Experienced microbiologists familiar with the views of the most common micro-organisms under the microscope, as well as the nature of their colonies on agar plates;they can often be identified isolated colonies of microorganisms without further testing.